期刊
THERANOSTICS
卷 7, 期 5, 页码 1114-1132出版社
IVYSPRING INT PUBL
DOI: 10.7150/thno.18175
关键词
DDX3; YAP1; SIX2; KRAS; and colorectal cancer
资金
- Ministry of Science and Technology in Taiwan, ROC [105-2320-B-038 -057, 105-2325-B-038 -003 -CC2]
The mechanism underlying tumor aggressiveness and cetuximab (CTX) resistance in KRAS-wild-type (KRAS-WT) colorectal cancer remains obscure. We here provide evidence that DDX3 promoted soft agar growth and invasiveness of KRAS-WT cells, as already confirmed in KRAS-mutated cells. Mechanistically, increased KRAS expression induced ROS production, which elevated HIF-1 alpha and YAP1 expression. Increased HIF-1 alpha persistently promoted DDX3 expression via a KRAS/ROS/HIF-1 alpha feedback loop. DDX3-mediated aggressiveness and CTX resistance were regulated by the YAP1/SIX2 axis in KRAS-WT cells and further confirmed in animal models. Kaplan-Meier and Cox regression analysis indicated that DDX3, KRAS, and YAP1 expression had prognostic value for OS and RFS in KRAS-WT and KRAS-mutated tumors, but SIX2 and YAP1/SIX2 were prognostic value only in KRAS-WT patients. The observation from patients seemed to support the mechanistic action of cell and animal models. We therefore suggest that combining YAP1 inhibitors with CTX may therefore suppress DDX3-mediated tumor aggressiveness and enhance CTX sensitivity in KRAS-WT colorectal cancer.
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