期刊
STEM CELL RESEARCH & THERAPY
卷 8, 期 -, 页码 -出版社
BIOMED CENTRAL LTD
DOI: 10.1186/s13287-017-0688-x
关键词
Aging; Stem cell markers; Cellular senescence; Senescence-associated secretory phenotype; Extracellular matrix; Stem cell niche
资金
- VA [1 I01 BX002145 01]
- Owens Medical Research Foundation
- VA [976122, 5I01BX002145-04] Funding Source: Federal RePORTER
Background: Degenerative diseases are a major public health concern for the aging population and mesenchymal stem cells (MSCs) have great potential for treating many of these diseases. However, the quantity and quality of MSCs declines with aging, limiting the potential efficacy of autologous MSCs for treating the elderly population. Methods: Human bone marrow (BM)-derived MSCs from young and elderly donors were obtained and characterized using standard cell surface marker criteria (CD73, CD90, CD105) as recommended by the International Society for Cellular Therapy (ISCT). The elderly MSC population was isolated into four subpopulations based on size and stage-specific embryonic antigen-4 (SSEA-4) expression using fluorescence-activated cell sorting (FACS), and subpopulations were compared to the unfractionated young and elderly MSCs using assays that evaluate MSC proliferation, quality, morphology, intracellular reactive oxygen species, beta-galactosidase expression, and adenosine triphosphate (ATP) content. Results: The ISCT-recommended cell surface markers failed to detect any differences between young and elderly MSCs. Here, we report that elderly MSCs were larger in size and displayed substantially higher concentrations of intracellular reactive oxygen species and beta-galactosidase expression and lower amounts of ATP and SSEA-4 expression. Based on these findings, cell size and SSEA-4 expression were used to separate the elderly MSCs into four subpopulations by FACS. The original populations (young and elderly MSCs), as well as the four subpopulations, were then characterized before and after culture on tissue culture plastic and BM-derived extracellular matrix (BM-ECM). The small SSEA-4-positive subpopulation representing similar to 8% of the original elderly MSC population exhibited a youthful phenotype that was similar to that of young MSCs. The biological activity of this elderly subpopulation was inhibited by senescence-associated factors produced by the unfractionated parent population. After these youthful cells were isolated and expanded (three passages) on a young microenvironment (i.e., BM-ECM produced by BM cells from young donors), the number of cells increased approximate to 17,000-fold to 3 x 10(9) cells and retained their youthful phenotype. Conclusions: These results suggest that it is feasible to obtain large numbers of high-quality autologous MSCs from the elderly population and establish personal stem cell banks that will allow serial infusions of rejuvenated MSCs for treating age-related diseases.
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