期刊
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
卷 41, 期 3, 页码 1135-1146出版社
Cell Physiol Biochem Press GmbH & Co
DOI: 10.1159/000464120
关键词
miR-543; RKIP; Prostate cancer; Metastasis; Proliferation
资金
- Application and Basic Research Project Of Wuhan City [2015060101010049]
- Hubei Province Health and Family Planning Scientific Research Project [WJ2017M025, WJ2017Z005]
- Bureau of Public Health of Hubei Province [JX6B62]
Background/Aims: MicroRNAs (miRNAs, miRs) have emerged as important post transcriptional regulators in various cancers. miR-543 has been reported to play critical roles in hepatocellular carcinoma and colorectal cancer, however, the role of miR-543 in the pathogenesis of prostate cancer has not been fully understood. Methods: Expression of miR-543 and Raf Kinase Inhibitory Protein (RKIP) in clinical prostate cancer specimens, two prostate cancer cell lines, namely LNCAP and C4-2B, were determined. The effects of miR-543 on proliferation and metastasis of tumor cells were also investigated with both in vitro and in vivo studies. Results: miR-543 was found to be negatively correlated with RKIP expression in clinical tumor samples and was significantly upregulated in metastatic prostate cancer cell line C4-2B compared with parental LNCAP cells. Further studies identified RKIP as a direct target of miR-543. Overexpression of miR-543 downregulated RKIP expression and promoted the proliferation and metastasis of cancer cells, whereas knockdown of miR-543 increased expression of RKIP and suppressed the proliferation and metastasis of cancer cells in vitro and in vivo. Conclusion: Our study demonstrates that miR-543 promotes the proliferation and metastasis of prostate cancer via targeting RKIP. (C) 2017 The Author(s)
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