期刊
SCIENTIFIC REPORTS
卷 7, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-017-03630-y
关键词
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资金
- Jane and Aatos Erkko Foundation
- National Institute of General Medical Sciences of the National Institute of Health [P41 GM103445]
- US Department of Energy, Biological and Environmental Research [DE-AC02-05CH11231]
- Academy of Finland [138388, 259725]
- European Community's Seventh Framework programme [NMP.2013.1.4-1, 604005]
- Academy of Finland (AKA) [259725, 138388, 259725, 138388] Funding Source: Academy of Finland (AKA)
Various types of DNA viruses are known to elicit the formation of a large nuclear viral replication compartment and marginalization of the cell chromatin. We used three-dimensional soft x-ray tomography, confocal and electron microscopy, combined with numerical modelling of capsid diffusion to analyse the molecular organization of chromatin in herpes simplex virus 1 infection and its effect on the transport of progeny viral capsids to the nuclear envelope. Our data showed that the formation of the viral replication compartment at late infection resulted in the enrichment of heterochromatin in the nuclear periphery accompanied by the compaction of chromatin. Random walk modelling of herpes simplex virus 1-sized particles in a three-dimensional soft x-ray tomography reconstruction of an infected cell nucleus demonstrated that the peripheral, compacted chromatin restricts viral capsid diffusion, but due to interchromatin channels capsids are able to reach the nuclear envelope, the site of their nuclear egress.
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