期刊
EUROPEAN JOURNAL OF IMMUNOLOGY
卷 47, 期 6, 页码 1040-1050出版社
WILEY
DOI: 10.1002/eji.201746974
关键词
DTPiP vaccine; Influenza vaccine; NK cells; NKG2C; Vaccination
类别
资金
- U.K. Medical Research Council (MRC) [G1000808]
- U.K. Department for International Development (DFID) under the MRC/DFID Concordat agreement [G1000808]
- MRC [G1000808, MC_U123292701, MR/P012019/1] Funding Source: UKRI
- Medical Research Council [MC_U123292701, G1000808, MR/P012019/1] Funding Source: researchfish
Human cytomegalovirus (HCMV) infection drives the phenotypic and functional differentiation of NK cells, thereby influencing the responses of these cells after vaccination. NK cell functional differentiation is particularly advanced in African populations with universal exposure to HCMV. To investigate the impact of advanced differentiation on vaccine-induced responses, we studied NK-cell function before and after vaccination with Trivalent Influenza Vaccine (TIV) or diphtheria, tetanus, pertussis, inactivated poliovirus vaccine (DTPiP) in Africans with universal, lifelong HCMV exposure. In contrast to populations with lower prevalence of HCMV infection, no significant enhancement of NK-cell responses (IFN-gamma, CD107a, CD25) occurred after in vitro re-stimulation of post-vaccination NK cells with TIV or DTPiP antigens compared to pre-vaccination baseline cells. However, both vaccinations resulted in higher frequencies of NK cells producing IFN-gamma in response to exogenous IL-12 with IL-18, which persisted for up to 6 months. Enhanced cytokine responsiveness was restricted to less differentiated NK cells, with increased frequencies of IFN-gamma(+) cells observed within CD56(bright)CD57(-), CD56(dim)CD57(-)NKG2(-) and CD56(dim)CD57(-)NKG2C(+) NK-cell subsets. These data suggest a common mechanism whereby different vaccines enhance NK cell IFN-gamma function in HCMV infected donors and raise the potential for further exploitation of NK cellpre-activation to improve vaccine effectiveness.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据