期刊
ANTICANCER RESEARCH
卷 37, 期 6, 页码 2913-2918出版社
INT INST ANTICANCER RESEARCH
DOI: 10.21873/anticanres.11644
关键词
HPV16; inflammation; miRNAs; celecoxib; carcinogenesis
类别
资金
- Liga Portuguesa Contra o Cancro
- Research Center of the Portuguese Institute of Oncology of Porto [CI-IPOP 37-2016]
- Laboratory for Process Engineering, Environment, Biotechnology and Energy - LEPABE [POCI-01-0145-FEDER-006939]
- FEDER through COMPETE2020 - Programa Operacional Competitividade e Internacionalizacao (POCI)
- FCT, Fundacao paraa Ciencia e a Tecnologia
- FCT [SFRH/BPD/85462/2012]
- Portuguese Government
- Social European Fund
- [POCI-01-0145-FEDER-006958]
- [UID/AGR/04033/2013]
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/85462/2012, UID/AGR/04033/2013] Funding Source: FCT
Background/Aim: Human papillomavirus type 16 (HPV16) induces various types of cancer in several locations. Microenvironmental microRNAs (miRNAs) such as miRNA-146a and miRNA-150 regulate cancer-associated inflammation and are involved in HPV-induced carcinogenesis. We studied the effects of celecoxib on the expression of these two miRNAs in HPV16-induced lesions. Materials and Methods: Female transgenic (HPV16(+/-)) and wild-type (HPV16(-/-)) mice were administered 75 mg/kg/day celecoxib orally (treatment groups) or placebo (control groups) for four weeks. Skin samples were classified histologically, or used for miRNA analysis by quantitative real-time PCR. Results: HPV16(+/-) mice showed higher miRNA-146a and miRNA-150 expression levels compared to wild-type animals. Celecoxib further increased miRNA-150 (p<0.05) and miRNA-146a levels in treated animals. Celecoxib-treated HPV16(+/-) animals also showed reduced incidence of epidermal dysplasia and reduced inflammation, compared to untreated mice. Conclusion: In this model, celecoxib may be able to regulate tumour-associated inflammation, through mechanisms involving the regulation of miRNA-146a and miRNA-150.
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