期刊
MATERIALS
卷 10, 期 6, 页码 -出版社
MDPI AG
DOI: 10.3390/ma10060643
关键词
alkyl chain group; QAMs; antibacterial; demineralization
类别
资金
- International Science and Technology Cooperation Program of China [2014DFE30180]
- National Natural Science Foundation of China [81372889, 81430011, 81372890]
- Youth Grant of the Science and Technology Department of Sichuan Province, China [2017JQ0028]
- Innovative Research Team Program of Sichuan Province
- NIH [R01 DE17974]
- University of Maryland School of Dentistry bridge fund
The objectives of this study were to investigate the effects of dental adhesives containing quaternary ammonium methacrylates (QAMs) with different alkyl chain lengths (CL) on ecological caries prevention in vitro. Five QAMs were synthesized with a CL = 3, 6, 9, 12, and 16 and incorporated into adhesives. Micro-tensile bond strength and surface charge density were used to measure the physical properties of the adhesives. The proportion change in three-species biofilms consisting of Streptococcus mutans, Streptococcus sanguinis, and Streptococcus gordonii was tested using the TaqMan real-time polymerase chain reaction. Lactic acid assay, MTT [3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, exopolysaccharide staining, live/dead staining, scanning electron microscopy (SEM), and transverse microradiography (TMR) were performed to study the anti-biofilm and anti-demineralization effects of the dental adhesives. The results showed that incorporating QAMs with different alkyl chain lengths into the adhesives had no obvious effect on the dentin bond strength. The adhesives containing QAMs with a longer alkyl chain developed healthier biofilms. The surface charge density, anti-biofilm, and anti-demineralization effects of the adhesives increased with a CL of the QAMs from 3 to 12, but decreased slightly with a CL from 12 to 16. In conclusion, adhesives containing QAMs with a tailored chain length are promising for preventing secondary caries in an ecological way.
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