期刊
BIOFABRICATION
卷 9, 期 2, 页码 -出版社
IOP PUBLISHING LTD
DOI: 10.1088/1758-5090/aa74f2
关键词
three-dimensional bioprinting; laser printing; energy absorbing layer; gelatin; cell viability; DNA damage
资金
- US National Science Foundation [CMMI 1258536/1314830, 1537956]
- Directorate For Engineering
- Div Of Civil, Mechanical, & Manufact Inn [1537956, 1314830] Funding Source: National Science Foundation
Laser-induced forward transfer printing, also commonly known as laser printing, has been widely implemented for three-dimensional bioprinting due to its unique orifice-free nature during printing. However, the printing quality has the potential to be further improved for various laser bioprinting applications. The objectives of this study are to investigate the feasibility of using gelatin as an energy absorbing layer (EAL) material for laser bioprinting and its effects on the quality of printed constructs, bioink printability, and post-printing cell viability and process-induced DNA damage. The gelatin EAL is applied between the quartz support and the coating of build material, which is to be printed. Printing quality can be improved by EAL-assisted laser printing when using various alginate solutions (1%, 2%, and 4%) and cell-laden bioinks (2% alginate and 5 x 10(6) cells ml(-1) in cell culture medium). The required laser fluence is also reduced due to a higher absorption coefficient of gelatin gel, in particular when to achieve the best printing type/quality. The post-printing cell viability is improved by similar to 10% and DNA double-strand breaks are reduced by similar to 50%. For all the build materials investigated, the gelatin EAL helps reduce the droplet size and average jet velocity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据