期刊
CARBOHYDRATE POLYMERS
卷 165, 期 -, 页码 384-393出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2017.02.050
关键词
Antibiotics; Antimicrobial peptides; Azide-alkyne coupling; Biofilms; Chitosan; Click chemistry; CuAAC
资金
- Fundacao para a Ciencia e Tecnologia (FCT), Portugal [UID/Multi/04378/2013, SFRH/BD/108966/2015]
- Comissao de Coordenacao e Desenvolvimento Regional do Norte ((CCDR-N)/NORTE/Portugal)
- Fundação para a Ciência e a Tecnologia [SFRH/BD/108966/2015] Funding Source: FCT
Antimicrobial peptides (AMP) are promising alternatives to classical antibiotics, due to their high specificity and potency at low concentrations, and low propensity to elicit pathogen resistance. Immobilization of AMP onto biomaterials is an emergent field of research, towards creation of novel antimicrobial materials able to avoid formation of biofilms on the surfaces of medical devices. Herein, we report the chemical route towards one such material, where chitosan was used as biocompatible carrier for the covalent grafting of Dhvar-5, a well-known potent AMP, via the chemoselective (click) Cu(I)-catalyzed azidealkyne cycloaddition (CuAAC). The material's structure, as well as peptide loading, were confirmed by Fourier-transformed infra-red (FT-IR) and X-ray photoelectron (XPS) spectroscopies, and by Amino Acid Analysis (AAA), respectively. Results herein reported demonstrate that, with proper optimization, the click CuAAC is an attractive approach for the tethering of AMP onto chitosan, in order to create novel antimicrobial materials potentially valuable for biomedical applications. (C) 2017 Elsevier Ltd. All rights reserved.
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