期刊
SCIENTIFIC REPORTS
卷 7, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-017-05174-7
关键词
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资金
- CPRIT predoctoral cancer intervention and prevention discovery training program fellowship [RP140110]
- NIH/NCI [K99/R00, CA197672-01]
- National Cancer Institute [Lung SPORE P50CA70907]
- US National Institute of Aging [AG01228]
- Simmons NCI Comprehensive Cancer Center Support Grant [CA142543]
- distinguished Southland Financial Corporation Chair in Geriatrics Research
- National Institute of Health grant [C06 RR30414]
Telomerase activity is not readily detected in resting human T lymphocytes, however upon antigen presentation, telomerase is transiently upregulated. Presently, it is not known if telomerase activation is necessary for the proliferation of T cells or for the maintenance of telomere lengths. In this study, we found that telomerase activation is not required for the short-term proliferation of T cells and that telomeres progressively shorten in a heterogeneous population of T cells, even if telomerase is detected. By measuring telomerase activity at the single-cell level using quantitative ddPCR techniques (ddTRAP) and by monitoring changes in the shortest telomeres with more sensitive telomere length measurement assays, we show that only a subset of CD28+ T-cells have robust telomerase activity upon stimulation and are capable of maintaining their telomere lengths during induced proliferation. The study of this T-cell subset may lead to a better understanding on how telomerase is regulated and functions in immune cells.
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