期刊
SCIENTIFIC REPORTS
卷 7, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-017-02349-0
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资金
- Institut National de la Sante Et de la Recherche Medicale
- Universite Grenoble-Alpes
- Centre National de la Recherche Scientifique
- Laboratoire d'Excellence GRAL [ANR-10-LABX-49-01]
- Laboratoire d'Excellence SIGNALIFE [ANR-11-LABX-28-01]
- Agence National de la Recherche [ANR-15-CE11-0018-01]
- FRISBI within the Grenoble Partnership for Structural Biology (PSB) [ANR-10-INSB-05-02]
- GRAL within the Grenoble Partnership for Structural Biology (PSB) [ANR-10-LABX-49-01]
- Rhone-Alpes Region
- Fondation Recherche Medicale (FRM)
- fonds FEDER
- Centre National de la Recherche Scientifique (CNRS)
- University of Grenoble
- EMBL
- GIS-Infrastrutures en Biologie Sante et Agronomie (IBISA)
- Commissariat a l'Energie Atomique
Exolysin (ExlA) is a recently-identified pore-forming toxin secreted by a subset of Pseudomonas aeruginosa strains identified worldwide and devoid of Type III secretion system (T3SS), a major virulence factor. Here, we characterized at the ultrastructural level the lesions caused by an ExlA-secreting strain, CLJ1, in mouse infected lungs. CLJ1 induced necrotic lesions in pneumocytes and endothelial cells, resulting in alveolo-vascular barrier breakdown. Ectopic expression of ExlA in an exlA-negative strain induced similar tissue injuries. In addition, ExlA conferred on bacteria the capacity to proliferate in lungs and to disseminate in secondary organs, similar to bacteria possessing a functional T3SS. CLJ1 did not promote a strong neutrophil infiltration in the alveoli, owing to the weak pro-inflammatory cytokine reaction engendered by the strain. However, CLJ1 was rapidly eliminated from the blood in a bacteremia model, suggesting that it can be promptly phagocytosed by immune cells. Together, our study ascribes to ExlA-secreting bacteria the capacity to proliferate in the lung and to damage pulmonary tissues, thereby promoting metastatic infections, in absence of substantial immune response exacerbation.
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