期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 487, 期 4, 页码 923-929出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2017.05.005
关键词
lncRNA; Gm4419; Microglial cells; OGD/R; NF-kappa B pathway
资金
- GuiZhou Province Scientific Award for Society Development [SY[2012] 3118]
Ischemic stroke is one of major causes of adult morbidity. Recent studies have shown that over-activated microglial cells play a critical role in aggravating cerebral oxygen glucose deprivation/reoxygenation (OGD/R) damage by releasing excessive inflammatory cytokines. However, the involving mechanisms are not distinct yet. Long non-coding RNAs (lncRNAs) have been reported to in participate in lots of complicated biological processes. Our understandings of the relationship between lncRNAs and OGD/R injury are largely limited. In this study, we demonstrated that a lncRNA Gm4419 functioned as a crucial mediator in the activation of NF-kappa B signaling pathway, causing neuroinflammation damage during OGD/R. Gm4419 was abnormally up-regulated in OGD/R-treated microglial cells. We found that the high level of Gm4419 promoted the phosphorylation of I kappa B alpha by physically associating with I kappa B alpha, therefore, led to increased nucleus NF-kappa B levels for the transcriptional activation of TNE-alpha, IL-1 beta and IL-6. In addition, we also demonstrated that knockdown of Gm4419 functioned as NF-kappa B inhibitor in OGD/R microglial cells, showing that down-regulation of Gm4419 had protective role against OGD/R injury. In summary, Gm4419 is required for microglial cell OGD/R injury though the activation of NF-kappa B signaling. Thus, Gm4419 appears to be a promising therapeutic target for ischemic stroke. (C) 2017 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据