期刊
CHEMBIOCHEM
卷 18, 期 10, 页码 910-913出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201700023
关键词
acetaminophen; hydrogen peroxide; liquid chromatography; mass spectrometry; synthetic biomarker
资金
- AMED-CREST from the Japan Agency for Medical Research and Development, AMED
- Japan Society for the Promotion of Science (JSPS) [JP15J03449]
- Yamagata prefectural government
- City of Tsuruoka
- JSPS
- Grants-in-Aid for Scientific Research [16H02485, 15J03449] Funding Source: KAKEN
The use of synthetic biomarkers is an emerging technique to improve disease diagnosis. Here, we report a novel design strategy that uses analyte-responsive acetaminophen (APAP) to expand the catalogue of analytes available for synthetic biomarker development. As proof-of-concept, we designed hydrogen peroxide (H2O2)-responsive APAP (HR-APAP) and succeeded in H2O2 detection with cellular and animal experiments. In fact, for blood samples following HR-APAP injection, we demonstrated that the plasma concentration ratio [APAP+APAP conjugates]/[HR-APAP] accurately reflects in vivo differences in H2O2 levels. We anticipate that our practical methodology will be broadly useful for the preparation of various synthetic biomarkers.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据