期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 23, 期 29, 页码 7113-7124出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201700601
关键词
fluorine; helical structures; NMR spectroscopy; nucleic acids; RNA
资金
- Academy of Finland [251539, 256214]
- Academy of Finland (AKA) [251539, 256214, 256214, 251539] Funding Source: Academy of Finland (AKA)
Triplex-forming peptide nucleic acids (TFPNAs) were targeted to double-helical regions of F-19-labeled RNA hairpin models (a UA-rich duplex with a hexaethylene glycol (heg) loop and a microRNA model, miR-215). In addition to conventional UV-and circular dichroism (CD)-based detection, binding was monitored by F-19 NMR spectroscopy. Detailed information on the stoichiometry and transition between the triple-helical peptide nucleic acid (PNA)/RNA and PNA)(2)/RNA binding modes could be obtained. gamma-(R)-Hydroxymethyl- modified thymine-1-yl- and 2-aminopyridin-3-yl-acetyl derivatives of TFPNAs were additionally synthesized, which were targeted to the same RNA models, and the effect of the gamma-(R)-hydroxymethyl group on binding was studied. An appropriate pattern of gamma-(R)-hydroxymethyl modifications reduced the stability of the ternary complex and preferred stoichiometric binding to the miR-215 model.
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