期刊
CYTOMETRY PART A
卷 91A, 期 5, 页码 519-526出版社
WILEY
DOI: 10.1002/cyto.a.23081
关键词
intracellular vesicular traffic; quantitative phase imaging; tomography; microscopy; dynamic light scattering; interferometric imaging
资金
- National Science Foundation [CBET-0939511 STC]
- DBI [14-50962 EAGER]
- Direct For Biological Sciences
- Div Of Biological Infrastructure [1450962] Funding Source: National Science Foundation
- Directorate For Engineering
- Div Of Industrial Innovation & Partnersh [1353368] Funding Source: National Science Foundation
Due to the limitations of fluorescence imaging techniques, the study of intracellular cargo is typically restricted to two-dimensional analyses. To overcome low light levels and the risk of phototoxicity, we employ quantitative phase imaging, a family of full-field imaging techniques that measure the optical path length shift introduced by the specimen. Specifically, we use spatial light interference microscopy (SLIM) to study the transport of mass in whole tomographic volumes and show that a time-correlation technique, dispersion-relation phase spectroscopy (DPS), can be used to simultaneously assay the horizontal and vertical traffic of mass through a cell. To validate our method, we compare the traffic inside cell bodies and neuronal extensions, showing that the vertical transport of mass may prove a more sensitive and interesting metric than similar measurements limited to a 2D, horizontal plane. (C) 2017 International Society for Advancement of Cytometry
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