4.6 Article

Superparamagnetic iron oxide nanoparticles functionalized by peptide nucleic acids

期刊

RSC ADVANCES
卷 7, 期 25, 页码 15500-15512

出版社

ROYAL SOC CHEMISTRY
DOI: 10.1039/C7RA00519A

关键词

-

资金

  1. CARIPLO Foundation [2013-0752]

向作者/读者索取更多资源

A novel efficient method has been developed for covalently linking Peptide Nucleic Acid (PNA) oligomers and superparamagnetic iron oxide nanoparticles (SPION), to produce water soluble hybrid nanomaterials that can act as MRI contrast agents, as hyperthermia promoters and as PNA carriers. The multistep procedure involves: (i) preparation of oleate-stabilized SPION by using the thermal decomposition method, to control the size of the magnetic core (here 17 +/- 2 nm, by TEM measurement); (ii) exchange of the oleate layer by dimercaptosuccinic acid (DMSA), to impart water solubility and to provide functional groups for PNA grafting; (iii) functionalization of a PNA oligomer with a terminal maleimide moiety, to allow SPION-PNA conjugation by thiol-maleimide Michael addition reaction, exploiting the SH groups of DMSA on the SPION surface. The method was tested using a model PNA decamer containing all four nucleobases (-CTAGATCACT-). SPION-PNA conjugation by SH addition was found more efficient than conjugation through amide bond between the COOH groups of DMSA and the terminal NH2 groups of PNA. Elemental analysis, UV-Vis and IR spectra, and zeta-potential measures confirmed the PNA binding (a loading of ca. 400 PNA strands per SPION was estimated, molar ratio ca. 1 : 15 with respect to DMSA). A detailed characterization of the morphology, relaxivity and magnetic properties of the SPION used for PNA binding is reported, and compared to the one relative to the SPION-PNA conjugate. The analysis of the magnetic behaviour showed that the nanoparticles are in the superparamagnetic regime at room temperature, and have a considerably high saturation magnetization (85 emu g(-1)). Magnetic hyperthermia measurements gave a remarkable value of the heat released (SAR = 65 W g(-1)), which makes these SPION suitable for magnetic hyperthermia treatments. The SPION were also able to effectively shorten both longitudinal and transverse relaxation times of water, with r1 and r2 values higher with respect to routinely used commercial contrast agents, at the typical fields of clinical instrumentation.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据