期刊
RSC ADVANCES
卷 7, 期 24, 页码 14448-14460出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7ra00696a
关键词
-
资金
- MICINN [SAF2014-52632-R]
- FEDER
- Spanish Ministry of Education
- UK Engineering and Physical Sciences Research Council (EPSRC) [EP/H005625/1]
- EPSRC [EP/H005625/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/H005625/1] Funding Source: researchfish
Temperature-sensitive polymeric micelles were prepared from dextran grafted with poly(N-isopropylacrylamide) (PNIPAAm) or polyethylene glycol methyl ether (PEGMA) via controlled radical polymerization and evaluated as delivery systems of the anticancer drug methotrexate (MTX). Polymer-grafting was carried out after introduction of initiating groups onto the polysaccharide backbone, without the need for protection of hydroxyl groups and avoiding the use of toxic solvents. Temperature-responsive dextran-based copolymers were designed to exhibit self-aggregation behaviour, affinity for MTX and high cellular internalization. In addition, some grafted polymers incorporated 2-aminoethyl methacrylate to reinforce MTX encapsulation in the micelles by means of ionic interactions. Dextran-based micelles were cytocompatible and had an appropriate size to be used as drug carriers. MTX release was dependent on the pH and temperature. The combination of poly(2-aminoethylmethacrylate) and PNIPAAm with the dextran backbone permitted the complete release of MTX at normal physiological temperature. Co-polymer micelles were highly internalized by tumour cells (CHO-K1) and, when loaded with MTX, led to enhanced cytotoxicity compared to the free drug.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据