期刊
ANALYTICA CHIMICA ACTA
卷 975, 期 -, 页码 42-51出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2017.04.012
关键词
Matrix-assisted laser desorption/ionization; Mass spectrometric imaging; Umbelliferone matrix; Methamphetamine abuse; Segmental hair analysis
资金
- Found from Shanghai Key Laboratory of Forensic Medicine (Department of Forensic Toxicology, Institute of Forensic Sciences, Ministry of Justice) [KF1602]
- National Natural Science Foundation of China [21405104]
- Youth Initial Foundation of Shanghai Jiao Tong University [15X100040022]
Segmental hair analysis offers a longer period for retrospective drug detection than blood or urine. Hair is a keratinous fiber and is strongly hydrophobic. The embedding of drugs in hydrophobic hair at low concentrations makes it difficult for extraction and detection with matrix-assisted laser desorption/ionization (MALDI) coupled with mass spectrometric imaging (MSI). In this study, a single scalp hair was longitudinally cut with a cryostat section to a length of 4 mm and fixed onto a stainless steel MALDI plate. Umbelliferone was used as a new hydrophobic matrix to enrich and assist the ionization efficiency of methamphetamine in the hair sample. MALDI-Fourier transform ion cyclotron resonance (FTICR)-MS profiling and imaging were performed for direct detection and mapping of methamphetamine on the longitudinal sections of the single hair sample in positive ion mode. Using MALDI-MSI, the distribution of methamphetamine was observed throughout five longitudinally sectioned hair samples from a drug abuser. The changes of methamphetamine were also semi-quantified by comparing the ratios of methamphetamine/internal standard (I.S). This method improves the detection sensitivity of target drugs embedded in a hair matrix for imaging with mass spectrometry. The method could provide a detection level of methamphetamine down to a nanogram per milligram incorporated into hair. The results were also compared with the conventional high performance liquid chromatography -tandem mass spectrometry (HPLC-MS/MS) method. Changes in the imaging results over time by the MSI method showed good semi-quantitative correlation to the results from the HPLC-MS/MS method. This study provides a powerful tool for drug abuse control and forensic medicine analysis in a narrow time frame, and a reduction in the sample amount required. (C) 2017 Elsevier B.V. All rights reserved.
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