期刊
RSC ADVANCES
卷 7, 期 84, 页码 53270-53277出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7ra11483d
关键词
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资金
- Fundamental Research Funds for the Central Universities, China [15CX05009A]
- Shandong Provincial Key Project of Research and Development, China [2016GGX102017]
- Natural Science Foundation of Shandong Province, China [ZR2015BM003, ZR2014BQ012]
- National Natural Science Foundation of China [21176259, 21406269]
Microalgal lipid is being considered as an alternative source of omega-3 polyunsaturated fatty acids (omega-3 PUFAs); however, the extraction of omega-3 PUFA-rich algal lipid still needs further research. In this study, single enzyme and their combinations were screened based on their cell wall disrupting capability and lipid recovery yield, and economical and feasible extraction parameters were determined. Lipid recovery could reach 22.18 +/- 0.26 wt% when algae was treated with an 8 : 1 (w/w) algae/cocktailenzyme ratio and a 2 : 1 cellulase/lysozyme mass ratio at 50 degrees C under stirring for 5 h at a pH of 4.0, and the content of PUFAs was as high as 23.30%. In addition, alkaline pretreatment efficiently facilitates the degradation of algal cell wall in the enzymatic hydrolysis, and it has been verified that cell wall ruptures via two steps: swelling by a weak alkali pretreatment and decomposition by enzymatic hydrolysis. Furthermore, all the extraction operations can take place under facile conditions. These results imply that algal lipid extracted by a weak alkali pretreatment aiding enzymatic hydrolysis, is the most appropriate raw material for omega-3 PUFAs.
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