期刊
RSC ADVANCES
卷 7, 期 88, 页码 55668-55672出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7ra11159b
关键词
-
资金
- National Natural Science Foundation of China [21645003]
In this work, we developed a novel fluorescence polarization (FP) method for the sensitive detection of Cu2+ based on the chemical ligation of DNA by click chemistry and silica nanoparticles-assisted FP enhancement. A double-stranded DNA hybrid (P1P2) is used as an end-of-helix template with an alkyne group and an azide group at the end of two DNA strands, respectively. Cu2+ can be reduced to Cu+ by sodium ascorbate, and trigger the chemical ligation P1P2 through the Cu+-catalyzed azide-alkyne cycloaddition. A biotin-labeled P3 which can hybridize with P1 is introduced to displace the FAM-contained P2 from P1P2 duplex. Since P1 and P2 are linked after the Cu2+-triggered click chemistry, a P1P2P3 complex is formed which can be captured on the silica nanoparticle surface through the biotin-streptavidin interaction, thus achieving a high FP signal. In the absence of Cu2+, there is no ligation between P1 and P2. The released P2 is free and cannot be immobilized on the nanoparticle surface, resulting in a low FP. The specificity of click chemistry provides a high selectivity, and the nanoparticle-assisted FP enhancement provides a high sensitivity with a detection limit of 0.0178 mu M. This method also displays good assay performance in real water samples, which may be further used for environmental monitoring.
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