期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 56, 期 24, 页码 6833-6837出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201701892
关键词
biological species; biosensors; imprinting; mussel-inspired polymers; potentiometry
资金
- National Natural Science Foundation of China [21475148, 41576106, 41206087]
- Science and Technology Project of Yantai [2014ZH086]
- Youth Innovation Promotion Association of CAS [2014190]
- Taishan Scholar Program of Shandong Province [TS20081159]
Using sensors to quantify clinically relevant biological species has emerged as a fascinating research field due to their potential to revolutionize clinical diagnosis and therapeutic monitoring. Taking advantage of the wide utility in clinical analysis and low cost of potentiometric ion sensors, we demonstrate a method to use such ion sensors to quantify bioanalytes without chemical labels. This is achieved by combination of chronopotentiometry with a mussel-inspired surface imprinting technique. The biomimetic sensing method is based on a blocking mechanism by which the recognition reaction between the surface imprinted polymer and a bioanalyte can block the current-induced ion transfer of an indicator ion, thus causing a potential change. The present method offers high sensitivity and excellent selectivity for detection of biological analytes. As models, trypsin and yeast cells can be measured at levels down to 0.03 UmL(-1) and 50 CFUmL(-1), respectively.
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