期刊
ACS APPLIED MATERIALS & INTERFACES
卷 9, 期 23, 页码 19562-19568出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b02853
关键词
metal-organic framework; fast functionalization; glycopeptide enrichment; cell lysate; mass spectrometry
资金
- National Natural Science Foundation of China [21575007, 21322505, 21527809]
Cysteine-functionalized metal organic framework (MOP) was synthesized via a common and facile two-step method of in situ loading of Au nanoparticles on amino-deiivetl MOF followed by L-cysteine (Cys) immobilization. Owing to the large specific surface area and ultrahigh hydrophilicity of this nanocomposite, excellent performance was observed in the enrichment of N-linked glycopeptides in both model glycoprotein and HeLa cell lysate. By using this nanocomposite, 16 and 31 glycopeptides were efficiently extracted from digest of horseradish peroxidase (HRP) and human serum iminunoglobulin G (IgG), respectively. The short incubation time (S min), large binding capacity (150 mg/g, IgG digest to material), good selectivity (1:50, molar ratio of IgG and bovine serum albumin (BSA) digest), high recovery (over 80%), and low detection limit (1 frnol) ensure the effectiveness and robustness of MIL-101(NH2)@Au-Cys in complex HeLa cell lysate. As a result, 1123 N-glycosylation sites corresponding to 1069 N-glycopeptides and 614 N-glycoproteins were identified from the lysate. Compared with those of previously reported hydrophilic methOds, to our knowledge, it was the best result. This work paves a new way for fast functionalization of MOF and also provides a novel idea for material design in sample preparation, especially in glycoproteome and related analysis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据