期刊
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
卷 1864, 期 7, 页码 1195-1206出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamcr.2017.03.005
关键词
SUMOylation; Epigenetic program; M-phase entry; Spindle morphogenesis; DNA damage; Preimplantation development
资金
- National Natural Science Foundation of China [31171378]
- Fundamental Research Funds for the Central University [2014PY045]
Understanding the mechanisms underlying abnormal egg production and pregnancy loss is significant for human fertility. SENP7, a SUMO poly-chain editing enzyme, has been regarded as a mitotic regulator of heterochromatin integrity and DNA repair. Herein, we report the roles of SENP7 in mammalian reproductive scenario. Mouse oocytes deficient in SENP7 experienced meiotic arrest at prophase I and metaphase I stages, causing a substantial decrease of mature eggs. Hyperaceylation and hypomethylation of histone H3 and up-regulation of Cdcl4B/C accompanied by down-regulation of CyclinB1 and CyclinB2 were further recognized as contributors to defective M phase entry and spindle assembly in oocytes. The spindle assembly checkpoint activated by defective spindle morphogenesis, which was also caused by mislocalization and ubiquitylation-mediated proteasomal degradation of gamma-tubulin, blocked oocytes at meiosis I stage. SENP7-depleted embryos exhibited severely defective maternal zygotic transition and progressive degeneration, resulting in nearly no blastocyst production. The disrupted epigenetic landscape on histone H3 restricted Rad51C loading onto DNA lesions due to elevated HP1 alpha euchromatic deposition, and reduced DNA 5hmC challenged the permissive status for zygotic DNA repair, which induce embryo death. Our study pinpoints SENP7 as a novel determinant in epigenetic programming and major pathways that govern oocyte and embryo development programs in mammals. (C) 2017 Elsevier B.V. All rights reserved.
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