期刊
BIOSENSORS & BIOELECTRONICS
卷 92, 期 -, 页码 457-464出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.10.080
关键词
Signal amplification; Alkaline phosphatase; Ag-Au bimetallic; Catalytic oxidation of AA
类别
资金
- National Natural Science Foundation of China [21305042, 21375037, 21475043]
- Scientific Research Fund of Hunan Provincial Education Department [14B116]
- Science and Technology Department [14JJ4030, 13JJ2020]
- Opening Fund of State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University [2013017]
- Collaborative Innovation Center of New Chemical Technologies for Environmental Benignity and Efficient Resource Utilization
- Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province
In this work, a novel immunosensor was constructed based on the alkaline phosphatase (ALP) in situ generating an electroactive substance by enzymatic hydrolysis the inactive substrates. The new signal-amplified strategy for sensitive detection of HIgG was based on the catalytic oxidation of ALP-generated products, ascorbic acid (AA), using carbon-based Ag-Au bimetallic as the catalyst and signal enhancer. Through a sandwich reaction, ALP-Ab(2) bioconjugates were captured on the electrode surface and the amplified signal can be obtained as follows: the ALP catalyzed the inactive substrate L-ascorbic acid 2-phosphate (AAP) to in situ produce AA; AA as an electroactive product then can be directly electro-oxidized to generate electrochemical signal; At the same time, AA could be catalytic oxidized by Ag-Au bimetallic and resulted in the amplification of electrochemical signal; Finally, the oxidation of Ag on the Ag-Au bimetallic maybe further enhance the detection signal. The proposed immunosensor achieved good linear in the range of 0.005-100 ng mL(-1) with the detection limit of 0.0009 ng mL(-1) (S/N =3). The proposed immunosensor was successfully applied in the analysis of human IgG in real samples and got satisfied results. The present work demonstrates a general strategy for the design of multifunctional nanomaterials based on carbon-based bimetallic nanoparticles for different applications, such as biosensors, immunosensors and nanocatalysts.
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