期刊
CARBOHYDRATE POLYMERS
卷 166, 期 -, 页码 64-72出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2017.02.058
关键词
Porcine circovirus type 2 (PCV2); Vaccine; Adjuvant; Chitosan oligosaccharide; Conjugation
资金
- National Programs for High Technology Research and Development (863 Programs) [2014AA093604]
- National Natural Science Fund of China [31500747, 31570801]
- Postdoctoral Science Fund of China [2014M561069]
This study aimed to investigate the effect of chitosan oligosaccharide (COS) conjugation on the immunogenicity of porcine circovirus type-2 (PCV2) vaccine. Two conjugates (PCV2-COS-1 and PCV2-COS-2) were designed by covalent conjugation of an inactivated PCV2 vaccine with COS, and administered to C57BL/6 mice three times at two-week intervals. The results indicate that, as compared to PCV2 alone group, the PCV2-COS conjugates remarkably enhanced both humoral and cellular immunity against PCV2 by promoting T lymphocyte proliferation and initiating a mixed Th1/Th2 response, including the elevated production of PCV-2 specific antibodies and up-regulated secretion of inflammatory cytoldnes. Noticeably, the immunization with PCV2-COS-1 conjugate displayed similar or even better immune-stimulating effects than that by PCV2/ISA206 (a commercialized adjuvant) and showed no infection or pathological signs at injection sites of the mice. Presumably, the covalent linkage of PCV2 vaccine to COS might be a viable strategy to increase the efficacy against PCV2-associated diseases. (C) 2017 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据