期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 154, 期 -, 页码 1-9出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2017.02.035
关键词
Electrospun fiber; Mechanical tension; Osteogenic differentiation; Cytoskeleton; Cell polarization
资金
- Key Technologies Research and Development Program of Science and Technology Department of Sichuan Province [2015SZ0127]
- Fundamental Research Funds for the Central Universities [2012SCU4A12]
- National Natural Science Foundation of China [30973346, 51373138]
- Sichuan Province Youth Science and Technology Innovation Team [2016TD0026]
Mesenchymal stem cells (MSCs) are able to self-renew and differentiate into tissues of mesenchymal origin, making them to be significant for cell-based therapies, such as metabolic bone diseases and bone repair. Regulating the differentiation of MSCs is significant for bone regeneration. Electrospun fibers mimicking natural extracellular matrix (ECM), is an effective artificial ECM to regulate the behaviors and fates of MSCs. The aligned electrospun fibers can modulate polar cell pattern of bone mesenchymal stem cells, which leads to more obvious osteogenic differentiation. Apart from the topographic effect of electrospun fibers, mechanical cues can also intervene the cell behaviors. In this study, the osteogenic differentiation of rat bone mesenchymal stem cells was evaluated, which were cultured on aligned/random electrospun fiber mats materials under mechanical tension intervention. Scanning electron microscope and immune-fluorescent staining were used to directly observe the polarity changing of cellular morphology and cytoskeleton. The results proved that aligned electrospun fibers could be more conducive to promote osteogenic differentiation of rat bone mesenchymal stem cells and this promotion of osteogenic differentiation was enhanced by tension intervention. These results were correlated to the quantitative real-time PCR assay. In general, culturing rat bone mesenchymal stem cells on electrospun fibers under the intervention of mechanical tension is an effective way to mimic a more real cellular microenvironment. (C) 2017 Elsevier B.V. All rights reserved.
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