期刊
RSC ADVANCES
卷 7, 期 56, 页码 35169-35174出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7ra05723g
关键词
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资金
- National Natural Science Foundation of China [21471002, 51572004]
- Outstanding Youth Science Foundation of Anhui Polytechnic University [2015JQ01]
- Young and Middle-Aged Talent Program of Anhui Polytechnic University [2016BJRC001]
An effective strategy for enhancement of catalytic activity and stability of immobilized lipase by interfacial activation on Fe3O4@PVBC (Fe3O4@ polyvinylbenzyl chloride) nanoparticles was developed, which involved the fabrication of core-shelled Fe3O4@PVBC nanoparticles via an emulsion polymerization process and the subsequent immobilization of lipase in phosphate buffer. Due to the magnetic nature of Fe3O4 cores and the presence of chloromethyl-functionalized polymer shells, the Fe3O4@ PVBC nanoparticles were employed as valid magnetic carriers for lipase immobilization. Bradford assays indicated that the loading amount of lipase on the Fe3O4@PVBC nanoparticles was calculated to be 162.5 mg protein per g particles. The catalytic activity of the immobilized lipase retained about 99.6 +/- 3.3% of the free enzyme activity, which was attributed to interfacial activation of lipase by Fe3O4@PVBC nanoparticles. Thermal and urea tolerance tests revealed that the immobilized lipase exhibited much better stabilities. Additionally, the immobilized lipase retained more than 69.8% of its initial activities after 10 times of reuse. It is believed that the results of the present investigation may provide a versatile approach for designing and fabricating biocatalysts with high activation and stability.
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