期刊
CHEMICAL COMMUNICATIONS
卷 53, 期 48, 页码 6448-6451出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7cc01483j
关键词
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资金
- National Natural Science Foundation of China [21422606, 21502189]
- Dalian Cultivation Fund for Distinguished Young Scholars [2014J11JH130, 2015J12JH205]
A 1,8-naphthalimide-derived fluorogenic probe was reported to label SNAP-tag fusion proteins in living cells. The probe can rapidly label a SNAP-tag and exhibit a fluorescence increase of 36-fold due to the additive effects of environment sensitivity of fluorophores and inhibition of photo-induced electron transfer from O-6-benzylguanine to the fluorophore. The labeling of intracellular proteins has been successfully achieved without a wash-out procedure.
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