期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 81, 期 7, 页码 1379-1385出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09168451.2017.1318695
关键词
proteoglycan; cell growth; Erk phosphorylation; normal human dermal fibroblast
类别
资金
- Regional Innovation Strategy Support Program
- Grants-in-Aid for Scientific Research [16H05185] Funding Source: KAKEN
Proteoglycan (PG) is a heavily glycosylated protein, localized to cell surface and extracellular matrix, and has various functions. Recently, it has been gradually revealed that PG interacts with various growth factors and morphogens and regulates cellular functions. Although salmon nasal cartilage PG (Salmon-PG) increases proliferation of immortalized cells, its mechanism remains unclear. In this study, we confirmed the effect of Salmon-PG on normal human dermal fibroblast (NHDF) and investigated the mechanism of PG action on NHDF. Salmon-PG dose- and time-dependently increased NHDF proliferation. Receptor tyrosine kinase array revealed that Salmon-PG increased only Erk1/2 signaling. Erk1/2 phosphorylation was significantly increased by Salmon-PG in a time-(10 min) and dose-(400 or 800 g/mL) dependent manner. MEK inhibitor suppressed the enhancement of NHDF proliferation by Salmon-PG. The overall findings indicate that Salmon-PG plays a role as a growth factor in NHDF via Erk1/2 activation, suggesting that Salmon-PG contributes to the maintenance of skin homeostasis.
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