期刊
ANALYTICAL CHEMISTRY
卷 89, 期 12, 页码 6794-6799出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b01218
关键词
-
资金
- NSF of China [21475138, 21322503, 21621062, 21435007, 21210007, 21475114, 21275123]
- National Basic Research Program of China (973 Programs) [2013CB933704, 2016YFA0200104]
- Chinese Academy of Sciences
Development of new principles and methods for cerebral ATP assay is highly imperative not only for determining ATP dynamics in brain but also for understanding physiological, and pathological processes related to ATP. Herein, we for the first time demonstrate that micrometer scale ion current rectification (MICR) at a polyimidazolium brush-modified micropipette can be used as the signal transduction output for the cerebral ATP assay with a high selectivity. The rationale for ATP assay is essentially based on the competitive binding ability between positively charged polyimidazolium and ATP toward negatively charged ATP aptamer. The method is well responsive to ATP with a good linearity within a concentration range from 5 nM to 100 nM, and high selectivity toward ATP. These properties essentially enable the method to determine the cerebral ATP by combining in vivo microdialysis. The basal dialysate level of ATP in rat brain cortex is determined to be 11.32 +/- 2.36 nM (n = 3). This study demonstrates that the MICR-based sensors could be potentially used for monitoring neurochemicals in cerebral systems.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据