期刊
ANALYTICAL CHEMISTRY
卷 89, 期 12, 页码 6854-6860出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b01351
关键词
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资金
- National Natural Science Foundation of China [21005068, 21475114, 21505113]
- Hunan Provincial Natural Science Foundation [2015JJ6104]
- China Postdoctoral Science Foundation [2014M552140, 2015T80876]
- State Key Laboratory of Chemo/Biosensing and Chemometrics Foundation [2015007]
- Scientific Research Fund of Hunan Provincial Education Department [15K125, 16B252]
- Hunan Collaborative Innovation Center of Chemical Engineering and Technology
- Environmental Benignity and Effective Resource Utilization, Hunan Provincial Innovation Foundation for Postgraduate
Alkaline phosphatase (ALP) is an essential enzyme and widely distributes in a variety of tissues. To date, various nanomaterial and small-molecule fluorescent probes for ALP have been constructed successfully, but the emission wavelengths of these probes are in the ultraviolet or visible range, which is not beneficial for bioimaging. Herein, a hemicyanine-based near-infrared (NIR) fluorescent probe named CyP is first synthesized and used to detect ALP activity. The characteristics of probe CyP are as follows: (1) The probe possesses a facile structure, which can be obtained by easy synthetic steps. (2) The fluorescence emission of the sensing system is at 738 nm belonging to NIR region, which is suitable for bioimaging in vivo. (3) The probe exhibits high sensitivity to ALP with 10-fold fluorescence enhancement and low detection limit (0.003 U/mL) can match the level of ALP in vivo. (4) The fluorescent change of the probe is attributed to the fact that ALP-catalyzed cleavage of the phosphate group in CyP induces the transformation of CyP (fluorescence off) into CyOH (fluorescence on), which is proved by HPLC, P-31 NMR, MS, and DFT calculation. (5) The NIR fluorescent probe is applied for the detection of endogenous ALP activity in various biological samples such as cell, tissue, and living animal with satisfactory results.
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