期刊
ANALYTICAL CHEMISTRY
卷 89, 期 12, 页码 6781-6786出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b01149
关键词
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资金
- BBSRC ZELS Grant [BB/ L018853/1]
- Francis Crick Institute
- Cancer Research UK [FC001030]
- UK Medical Research Council [FC001030]
- Wellcome Trust [FC001030]
- BBSRC [BBS/E/I/00001759, BB/I001824/1, BBS/E/I/00007039, BB/N002571/1, BB/L004240/1, BBS/E/I/00001422, BB/I001182/1, BB/L018853/1, BB/P025803/1, BBS/E/I/00001708, BBS/E/I/00007035, BB/L026341/1, BBS/E/I/00001981] Funding Source: UKRI
- MRC [MC_U117585868] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/I/00007035, BBS/E/I/00001981, BBS/E/I/00007039, BB/L018853/1, BB/L004240/1, BB/P025803/1, BB/I001182/1, BBS/E/I/00001422, BB/N002571/1, BBS/E/I/00001708, BBS/E/I/00001025, BB/L026341/1, BBS/E/I/00001759] Funding Source: researchfish
- Medical Research Council [MC_U117585868] Funding Source: researchfish
- The British Council [261727271] Funding Source: researchfish
- The Francis Crick Institute [10030] Funding Source: researchfish
Different influenza virus strains have caused a number of recent outbreaks killing scores of people and causing significant losses in animal farming. Simple, rapid, sensitive, and specific detection of particular strains, such as a pandemic strain versus a previous seasonal influenza, plays a crucial role in the monitoring, controlling, and management of outbreaks. In this paper we describe a dual recognition element lateral flow assay (DRELFA) which pairs a nucleic acid aptamer with an antibody for use as a point-of-care platform which can detect particular strains of interest. The combination is used to overcome the individual limitations of antibodies' cross-reactivity and aptamers' slow binding kinetics. In the detection of influenza viruses, we show that DRELFA can discriminate a particular virus strain against others of the same subtype or common respiratory diseases while still exhibiting fast binding kinetic of the antibody-based lateral flow assay (LFA). The improvement in specificity that DRELFA exhibits is an advantage over the currently available antibody-based LFA systems for influenza viruses, which offer discrimination between influenza virus types and subtypes. Using quantitative real-time PCR (qRT-PCR), it showed that the DRELFA is very effective in localizing the analyte to the test line (consistently over 90%) and this is crucial for the sensitivity of the device. In addition, color intensities of the test lines showed a good correlation between the DRELFA and the qRT-PCR over a 50-fold concentration range. Finally, lateral flow strips with a streptavidin capture test line and an anti-antibody control line are universally applicable to specific detection of a wide range of different analytes.
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