期刊
ANALYTICA CHIMICA ACTA
卷 973, 期 -, 页码 82-90出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2017.03.048
关键词
ZnO nanorod; Photoelectrochemical immunosensor; Nanobody; NDPK-A
资金
- National Natural Science Foundation of China [21505018]
- Natural Science Foundation of Jiangsu Province of China [BK 20141348]
- Fundamental Research Funds for the Central Universities
Nucleoside diphosphatase kinase A (NDPK-A) is a metastasis-suppressor protein and a biomarker that act on a wide range cancer cells to inhibit the potential metastasis. Herein, we present a simple photoelectrochemical immunosensor based on ZnO nanorod arrays for the sensitive detection of NDPK-A. The ZnO nanorod arrays cosensitized with CdS nanoparticles and Mn-2 broken vertical bar displayed a high and stable photocurrent response under irradiation. After anti-NPDK-A nanobodies were immobilized to the ZnO nanorod arrays, the proposed immunosensor can be utilized for detecting NPDK-A by monitoring the changes in the photocurrent signals of the electrode resulting from immunoreaction. Accordingly, the well-designed immunosensor exhibited a low limit of detection (LOD) of 0.3 pg mL(-1) and a wide linear range from 0.5 pg mL(-1) to 10 mg mL(-1). The R-2 of the regression curve is 0.99782. Meanwhile, the good stability, reproducibility and specificity of the resulting photoelectrochemical biosensor are demonstrated. In addition, the presented work would offer a novel and simple approach for the detection of immunoreactions and provide new insights in popularizing the diagnosis of NPDK-A. (C) 2017 Elsevier B.V. All rights reserved.
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