期刊
ACS APPLIED MATERIALS & INTERFACES
卷 9, 期 20, 页码 17638-17645出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b04553
关键词
electrochemiluminescence (ECL); DNA tetrahedron; programmable DNA cyclic amplification; multilayers; biosensors
资金
- Ministry of Science and Technology of China [2016YFA0201200]
- National Natural Science Foundation of China [21327902, 21535003]
- Natural Science Foundation of Jiangsu Normal University [16XLR012]
A novel DNA tetrahedron-structured electrochemiluminescence (ECL) platform for bioanalysis with programmable DNA cyclic amplification was developed. In this work, glucose oxidase (GOD) was labeled to a DNA sequence (5) as functional conjugation (GOD-S), which could hybridize with other DNA sequences (L and P) to form GOD-S:L:P probe. In the presence of target DNA and a help DNA (A), the programmable DNA cyclic amplification was activated and released GOD-S via toehold-mediated strand displacement. Then, the obtained GOD-S was further immobilized on the DNA tetrahedral scaffolds with a pendant capture DNA and Ru(bpy)(3)(2+)-conjugated silica nanopartides (RuSi NPs) decorated on the electrode surface. Thus, the amount of GOD-S assembled on the electrode surface depended on the concentration of target DNA and GOD could catalyze glucose to generate H2O2 in situ. The ECL signal of Ru(bpy)(3)(2+)-TPrA system was quenched by the presence of H2O2. By integrating the programmable DNA cyclic amplification and in situ generating H2O2 as Ru(bpy)(3)(2+) ECL quencher, a sensitive DNA tetrahedron -structured ECL sensing platform was proposed for DNA detection. Under optimized conditions, this biosensor showed a wide linear range from 100 aM to 10 pM with a detection limit of 40 aM, indicating a promising application in DNA analysis. Furthermore, by labeling GOD-to different recognition elements, the proposed strategy could be used for the detection of various targets. Thus, this programmable cascade amplification strategy not only retains the high selectivity and good capturing efficiency of tetrahedral-decorated electrode surface but also provides potential applications in the construction of ECL biosensor.
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