期刊
ONCOTARGET
卷 8, 期 42, 页码 71725-71735出版社
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.17847
关键词
gastric cancer; B7-H3; CXCR4; migration; invasion
资金
- National Natural Science Foundation of China [81672970, 8130193]
- Jiangsu Province's Clinical Medical Science and Technology Program [BL2013016]
- Provincial Natural Science Foundation of Jiangsu Province [BK20161225]
- Natural Science Foundation of Jiangsu Provincial University, China [13KJB320018]
- Postdoctoral Science Foundation Grant of China [2016M591913]
- Focus of Clinical Disease Treatment Technology Special Funds of Suzhou City [LCZX201505]
- Jiangsu Province's Graduate Student Research Innovation Project [KYLX16_0162]
- Science and Technology Program of Suzhou City, China [SYS201539]
- Second Affiliated Hospital of Soochow University Preponderant Clinic Discipline Group Project Funding
B7-H3 (B7 homologue 3, CD276) is a member of the B7 immunoregulatory family and promotes tumor progression. The present study demonstrated that B7-H3 promotes gastric cancer cell migration and invasion. shRNA-mediated B7-H3 silencing in the N87 gastric cancer cell line suppressed cell migration and invasion in vitro and in vivo; downregulated metastasis-associated CXCR4; and inhibited AKT, ERK, and Jak2/Stat3 phosphorylation. B7-H3-silenced cells injected into the tail veins of 4-week-old female BALB/c nude mice produced fewer metastases than control cells, and resulted in longer survival times. Immunofluorescence analyses confirmed B7H3/ CXCR4 colocalization in N87 cells, and co-immunoprecipitation assays showed a direct interaction between the two proteins. Our analysis of 120 tissue samples from gastric cancer patients showed that increased B7-H3 expression correlated positively with both tumor infiltration depth and CXCR4 expression. These findings suggest that B7-H3 and CXCR4 may be novel targets for anti-gastric cancer therapeutics.
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