期刊
ONCOTARGET
卷 8, 期 44, 页码 77360-77368出版社
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.20483
关键词
miR-30a; BECN1; autophagy; diabetic cataract; lens epithelial cells
资金
- National Natural Science Foundation of China [81370996, 81670839]
- Shandong Academy of Medical Sciences
Purpose: To investigate the role of microRNAs in the regulation of autophagy and apoptosis in lens epithelial cells (LECs) during diabetic cataract formation. Methods: A miRNA microarray study and quantitative real-time PCR were performed to identify the expression of miRNAs in LECs of diabetic cataract. Human LECs were cultured in high glucose conditions as a diabetic cataract model. BECN1 and LC3B were detected by Western blotting and quantitative real-time PCR. The extent of apoptosis was measured using FACSCalibur flow cytometry. Results: Downregulation of miR-30a was identified in LECs attached to diabetic cataract tissues. By the bioinformatic assay and the luciferase activity assay, BECN1 was found to be a direct target of miR-30a. MiR-30a reduced the BECN1-mediated autophagy activity induced by high glucose in LECs in vitro. The ratio of LECs apoptosis was also decreased. Conclusion: MiR-30a was involved in the inhibition of autophagy by targeting BECN1 in LECs in human diabetic cataract.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据