期刊
BIOMATERIALS
卷 133, 期 -, 页码 82-93出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2017.04.024
关键词
Dual-response; Dual-ratiometric; Quantification; SO2/ClO-; Imaging analysis
资金
- National Natural Science Foundation of China [21475074, 21403123, 21375075, 31301595, 21402106, 21475075, 21275089, 21405093, 21405094, 81303179, 21305076]
- Shandong Province Key Laboratory of Detection Technology for Tumor Markers [KLDTTM2015-6]
- Opening Foundation of Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers [KLDTTM2015-9]
- Taishan Scholar Foundation of Shandong Province, PR China
Intracellular reactive sulfur species and reactive oxygen species play vital roles in immunologic mechanism. As an emerging signal transmitter, SO2 can be generated as the anti-oxidant, while SO2 is also a potential oxidative stress-inducer in organism. Aiming to elucidate in-depth the dichotomous role of SO2 under oxidative stress, we designed a dual-response fluorescent probe that enabled the respective or successive detection of SO2 and ClO-. The probe itself emits the red fluorescence (625 nm) which can largely switch to blue (410 nm) and green fluorescence (500 nm) respectively in response to SO2 and ClO-, allowing the highly selective and accurate ratiometric quantification for both SO2 and ClO- in cells. Moreover the ultrafast (SO2: <60 s; ClO-: within sec) and highly sensitive (detection limits: SO2: 3.5 nM; ClO-: 12.5 nM) detection were achieved. With the robust applicability, the developed probe was successfully used to quantify SO2 and endogenous ClO- in respectively the HeLa cells and the RAW 264.7 cells, as well as to visualize the dynamic of SO2/ClO- in zebrafish. The fluorescent imaging studies and flow cytometry analysis confirmed the burst-and-depletion and meanwhile the oxidative-and-antioxidative effects of intracellular SO2 under the NaClO induced oxidative stress. (C) 2017 Elsevier Ltd. All rights reserved.
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