期刊
ONCOTARGET
卷 8, 期 33, 页码 54160-54172出版社
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.16952
关键词
tumour immunology; phosphopeptide; peptide-MHC complex; neoepitope; peptide conformation
资金
- CRUK project [C17422/A11740]
- Wellcome Trust New Investigator Award [099266/Z/12/Z]
- Medical Research Council PhD studentship
- Wellcome Trust [099266/Z/12/Z] Funding Source: Wellcome Trust
Dysregulated post-translational modification provides a source of altered selfantigens that can stimulate immune responses in autoimmunity, inflammation, and cancer. In recent years, phosphorylated peptides have emerged as a group of tumour-associated antigens presented by MHC molecules and recognised by T cells, and represent promising candidates for cancer immunotherapy. However, the impact of phosphorylation on the antigenic identity of phosphopeptide epitopes is unclear. Here we examined this by determining structures of MHC-bound phosphopeptides bearing canonical position 4-phosphorylations in the presence and absence of their phosphate moiety, and examining phosphopeptide recognition by the T cell receptor (TCR). Strikingly, two peptides exhibited major conformational changes upon phosphorylation, involving a similar molecular mechanism, which focussed changes on the central peptide region most critical for T cell recognition. In contrast, a third epitope displayed little conformational alteration upon phosphorylation. In addition, binding studies demonstrated TCR interaction with an MHC-bound phosphopeptide was both epitope-specific and absolutely dependent upon phosphorylation status. These results highlight the critical influence of phosphorylation on the antigenic identity of naturally processed class I MHC epitopes. In doing so they provide a molecular framework for understanding phosphopeptide-specific immune responses, and have implications for the development of phosphopeptide antigen-specific cancer immunotherapy approaches.
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