期刊
BIORESOURCE TECHNOLOGY
卷 236, 期 -, 页码 49-59出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.03.148
关键词
Lignocellulosic biomass; Fungal pretreatment; Supplements; Lignocellulolytic enzymes; Lignin degradation; Optimization
资金
- Ministry of Human Resource Development (MHRD), Govt. of India
The objective of this work was to study the increase in multiple lignolytic enzyme productions through the use of supplements in combination in pretreatment of sweet sorghum bagasse (SSB) by Coriolus versicolor such that enzymes act synergistically to maximize the lignin degradation and selectivity. Enzyme activities were enhanced by metallic salts and phenolic compound supplements in SSF. Supplement of syringic acid increased the activities of LiP, AAO and laccase; gallic acid increased MnP; CuSO4 increased laccase and PPO to improve the lignin degradations and selectivity individually, higher than control. Combination of supplements optimized by RSM increased the production of laccase, LiP, MnP, PPO and AAO by 17.2, 45.5, 3.5, 2.4 and 3.6 folds respectively for synergistic action leading to highest lignin degradation (2.3 folds) and selectivity (7.1 folds). Enzymatic hydrolysis of pretreated SSB yielded similar to 2.43 times fermentable sugar. This technique could be widely applied for pretreatment and enzyme productions. (C) 2017 Elsevier Ltd. All rights reserved.
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