期刊
ONCOTARGET
卷 8, 期 61, 页码 103671-103681出版社
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.22110
关键词
breast cancer; brain metastases; tumor infiltrating lymphocytes; PD-L1; immune microenvironment
资金
- Practical Research for Innovative Cancer Control from the Japan Agency for Medical Research and Development, AMED [15ck0106049h0002]
- National Cancer Center Research and Development Fund [26-A-4, 23-A-17]
- MEXT KAKENHI [26870597]
- JSPS KAKENHI [15H04796]
- Tokai University School of Medicine Research Aid
- Tokai University Educational System General Research Organization
- Grants-in-Aid for Scientific Research [26870597, 15H04796] Funding Source: KAKEN
Background: Immune checkpoint inhibitors are reported to be effective in patients with brain metastases. However, detailed characteristics of the brain metastasis immune microenvironment remain unexplored. Results: The median tumor-infiltrating lymphocyte (TIL) category in brain metastases was 5% (1-70%). In 46 pair-matched samples, the percentages of TILs were significantly higher in primary breast tumors than in brain metastases (paired t-test, P < 0.01). The numbers of CD4/CD8/Foxp3-positive cells were significantly higher in primary breast tumors than in brain metastases (paired t-test, P < 0.05 for all antibodies). In patients with triple-negative breast cancer specifically, low TIL numbers were associated with significantly shorter overall survival compared to high TIL numbers (log-rank test, P = 0.04). Materials and Methods: We retrospectively identified 107 patients with breast cancer and brain metastases who had undergone surgery between 2001 and 2012 at 8 institutions, and collected 191 samples including brain metastases alone and primary tumors with pair-matched brain metastasis samples. Hematoxylin and eosin-stained slides were evaluated for TILs and categorized according to the extent of staining. Immunohistochemistry for CD4, CD8, Foxp3, PD-L1, PD-L2, and HLA class I was also performed. Conclusions: There are significantly fewer TILs in brain metastases than in primary breast tumors.
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