期刊
ONCOTARGET
卷 8, 期 41, 页码 69722-69730出版社
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.19207
关键词
lncRNA; miRNA; gene regulation; Hirschsprung's disease
资金
- Natural Science Foundation of China [NSFC 81370473, NSFC 81400574, NSFC 81570467]
- school fund of Nanjing Medical University [2014NJMU145]
- State Key Laboratory of Reproductive Medicine [SKLRM-K201506]
The long noncoding RNA (lncRNA) MEG3 is involved in various biological processes including cell migration and cell proliferation. In present study, it was found that MEG3 and the intronic miR-770-5p were decreased in samples from HSCR patients. Besides, knockdown of MEG3 and miR-770-5p suppressed cell migration and proliferation, while cell cycle and apoptosis were not affected in human 293T and SH-SY5Y cells. SRGAP1 mRNA and protein upregulation was inversely correlated with miR-770-5p expression in tissue samples and cell lines, which was confirmed to be a target gene of miR-770-5p by dual-luciferase reporter assay. Moreover, silencing of SRGAP1 rescued the inhibition of cell migration and proliferation induced by MEG3 siRNA and miR-770-5p inhibition. The present study elucidates a novel mechanism of the development of HSCR and shows that the MEG3/miR-770-5p/SRGAP1 pathway plays a vital role in the pathogenesis of HSCR.
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