期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 74, 期 -, 页码 40-48出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2017.04.010
关键词
Chicken; IL-17B; NF-KB; Signal transduction; Cytokine
资金
- National Research Foundation of Korea - Korean government [NRF-2015R1D1A1A09059345]
- Chung-Ang University Excellent Student Scholarship, Republic of Korea
Interleukin (IL)-17B is a little known member of the IL -17 cytokine family, which plays an important role in immunity by regulating the expression of proinflammatory cytokines. In this study, we determined the coding sequence and biological functions of a novel chicken IL-17B (chlL-17B). The full-length chIL-17B coding sequence includes 567 nucleotides encoding 188 amino acids, which was identified in small intestinal epithelial cells. The chIL-17B protein shares 96.48% amino acid sequence identity with turkey, 92.57% with duck, and 44.92-64.06% with mammalian IL-17B proteins. ChIL-17B shares three exons and two introns with mammals, turkey, and duck. Moreover, IL-17B mRNA was more highly expressed than IL-17A mRNA in several organs of chickens infected with Salmonella and was upregulated in chicken cell lines following LPS stimulation. In addition, in chicken cell lines, chlL-17B induced the mRNA expression of several proinflammatory cytokines, including IL-1 beta, IL-6, LITAF, and INF-gamma, but not IL-17A, and activated MyD88, TAK1, NF-031, and SOCS1, which are associated with the NF-kappa B signaling pathway. Taken together, chicken interleukin-17B plays a critical role in host defense against the bacterial pathogens, and regulates proinflammatory cytokines by activating the NF-kappa B signaling pathway. (C) 2017 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据