期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 409, 期 17, 页码 4195-4205出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-017-0367-x
关键词
Anti-coagulant; Anti-thrombotic; Blacklip abalone; Viscera; Marine processing waste
资金
- International Postgraduate Research Scholarship (IPRS), Australia Postgraduate Award (APA) from the Australian Government
- Fisheries Research and Development Corporation (FRDC)
Abalone viscera contain sulphated polysaccharides with anti-thrombotic and anti-coagulant activities. In this study, a hydrolysate was prepared from blacklip abalone (Haliotis rubra) viscera using papain and bromelain and fractionated using ion exchange and size exclusion chromatography. Hydrolysates and fractions were investigated for in vitro thrombin inhibition mediated through heparin cofactor II (HCII) as well as anti-coagulant activity in plasma and whole blood. On the basis of sulphated polysaccharide concentration, the hydrolysate inhibited thrombin through HCII with an inhibitor concentration at 50% (IC50) of 16.5 mu g/mL compared with 2.1 mu g/mL for standard heparin. Fractionation concentrated HCII-mediated thrombin inhibition down to an IC50 of 1.8 mu g/mL and improved anti-coagulant activities by significantly delaying clotting time. This study confirmed the presence of anti-thrombotic and anti-coagulant molecules in blacklip abalone viscera and demonstrated that these activities can be enriched with a simple chromatography regime. Blacklip abalone viscera warrant further investigation as a source of nutraceutical or functional food ingredients.
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