期刊
ONCOTARGET
卷 8, 期 62, 页码 105525-105535出版社
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.22314
关键词
osteosarcoma; sphk2; microRNA-19a-3p; oncotarget
资金
- National Natural Science Foundation of China [81300955]
- Scientific Research Program of Nantong [MS22015047, MS12016009, wq2015011]
- Ke-Jiao-Qiang-Wei-Qing-Nian-Yi-Xue-Zhong-Dian-Ren-Cai Financial Assistance of Jiangsu Province [QNRC2016412]
It is needed to explore novel biological markers for early diagnosis and treatment of human osteosarcoma. Sphingosine kinase 2 (SphK2) expression and potential functions in osteosarcoma were studied. We demonstrate that SphK2 is over-expressed in multiple human osteosarcoma tissues and established human osteosarcoma cell lines. Silence of SphK2 by targeted-shRNAs inhibited osteosarcoma cell growth, and induced cell apoptosis. On the other hand, exogenous over-expression of SphK2 could further promote osteosarcoma cell growth. Notably, microRNA-19a-3p (miR-19a-3p) targets the 3' UTR (untranslated region) of SphK2 mRNA. Remarkably, forced-expression of miR-19a-3p silenced SphK2 and inhibited osteosarcoma cell growth. In vivo, SphK2 silence, by targeted-shRNA or miR-19a-3p, inhibited U2OS tumor growth in nude mice. These results suggest that SphK2 could be a novel and key oncotarget protein for OS cell progression.
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