期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 409, 期 17, 页码 4257-4266出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-017-0376-9
关键词
Amperometry; Urease; Enzyme inhibition; Metal ions; Lead and mercury
资金
- Department of Science & Technology, New Delhi [DST/ TM/WTI/2K14/197(a)(G), SR/FST/ETI-284/2011 (C), SR/FST/LSI-453/2010, SR/NM/PG-16/2007]
Intake of water contaminated with lead (Pb2+) and mercury (Hg2+) ions leads to various toxic effects and health issues. In this context, an amperometric urease inhibition-based biosensor was developed to detect Pb2+ and Hg2+ ions in water matrix. The modified Pt/CeO2/urease electrode was fabricated by immobilizing CeO2 nanoparticles and urease using a semi-permeable adsorption layer of nafion. With urea as a substrate, urease catalytic activity was examined through cyclic voltammetry. Further, maximum amperometric inhibitive response of the modified Pt/CeO2/urease electrode was observed in the presence of Pb2+ and Hg2+ ions due to the urease inhibition at specific potentials of -0.03 and 0 V, respectively. The developed sensor exhibited a detection limit of 0.019 +/- 0.001 mu M with a sensitivity of 89.2 x 10(-3) mu A mu M-1 for Pb2+ ions. A detection limit of 0.018 +/- 0.003 with a sensitivity of 94.1 x 10(-3) mu A mu M-1 was achieved in detecting Hg2+ ions. The developed biosensor showed a fast response time (< 1 s) with a linear range of 0.5-2.2 and 0.02-0.8 mu M for Pb2+ and Hg2+ ions, respectively. The modified electrode offered a good stability for 20 days with a good repeatability and reproducibility. The developed sensor was used to detect Pb2+ and Hg2+ ions contaminating Cauvery river water and the observed results were in good co-ordination with atomic absorption spectroscopic data.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据