期刊
NUTRIENTS
卷 9, 期 11, 页码 -出版社
MDPI AG
DOI: 10.3390/nu9111220
关键词
gut microbiota; humanized gnotobiotic mice; nonalcoholic fatty liver disease; high-fat diet
资金
- Ministry of Science and Technology (MOST) of Taiwan [MOST 106-2319-B-492-001]
- Ministry of Science and Technology of Taiwan [NSC 100-2320-B-492-001-, NSC 101-2320-B-492-001-MY3, MOST 106-2410-H-227-007-]
- Cathay General Hospital [CGH-MR-9820]
Nonalcoholic fatty liver disease (NAFLD) is a serious liver disorder associated with the accumulation of fat and inflammation. The objective of this study was to determine the gut microbiota composition that might influence the progression of NAFLD. Germ-free mice were inoculated with feces from patients with nonalcoholic steatohepatitis (NASH) or from healthy persons (HL) and then fed a standard diet (STD) or high-fat diet (HFD). We found that the epididymal fat weight, hepatic steatosis, multifocal necrosis, and inflammatory cell infiltration significantly increased in the NASH-HFD group. These findings were consistent with markedly elevated serum levels of alanine transaminase, aspartate transaminase, endotoxin, interleukin 6 (IL-6), monocyte chemotactic protein 1 (Mcp1), and hepatic triglycerides. In addition, the mRNA expression levels of Toll-like receptor 2 (Tlr2), Toll-like receptor 4 (Tlr4), tumor necrosis factor alpha (Tnf-alpha), Mcp1, and peroxisome proliferator-activated receptor gamma (Ppar-gamma) significantly increased. Only abundant lipid accumulation and a few inflammatory reactions were observed in group HL-HFD. Relative abundance of Bacteroidetes and Firmicutes shifted in the HFD-fed mice. Furthermore, the relative abundance of Streptococcaceae was the highest in group NASH-HFD. Nevertheless, obesity-related Lactobacillaceae were significantly upregulated in HL-HFD mice. Our results revealed that the gut microbiota from NASH Patients aggravated hepatic steatosis and inflammation. These findings might partially explain the NAFLD progress distinctly was related to different compositions of gut microbiota.
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