期刊
ANALYTICAL BIOCHEMISTRY
卷 530, 期 -, 页码 31-39出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2017.04.021
关键词
MALDI-ToF MS; Parylene-matrix chip; Newborn screening; Amino acid; Methanol extraction
资金
- National Research Foundation - Korean Government [2014M3A9E5073818]
- Korea Small and Medium Business Administration [S2220656]
- Korea Institute of Science and Technology (KIST) [2E26990]
- Korea Evaluation Institute of Industrial Technology (KEIT) [S2220656] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Ministry of Science & ICT (MSIT), Republic of Korea [2E26990] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Newborn screening for diagnosis of phenylketonuria, homocystinuria, and maple syrup urine disease have been conducted by analyzing the concentration of target amino acids using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS) based on parylene-matrix chip. Parylene-matrix chip was applied to MALDI-ToF MS analysis reducing the matrix peaks significantly at low mass-to-charge ratio range (m/z < 500). Reproducibility of inter-spot and intra-spot analyses of amino acids was less than 10%. Methanol extraction was adopted for simple and rapid sample preparation of serum before mass spectrometric analysis showing 13.3 to 45% of extraction efficiency. Calibration curves for diagnosis of neonatal metabolic disorders were obtained by analyzing methanol extracted serum spiked with target amino acids using MALDI-ToF MS. They showed good linearity (R-2 > 0.98) and the LODs were ranging from 9.0 to 22.9 mu g/mL. Effect of proteins in serum was estimated by comparing MALDI-ToF mass spectra of amino acids-spiked serum before and after the methanol extraction. Interference of other amino acids on analysis of target analyte was determined to be insignificant. From these results, MALDI-ToF MS based on parylene-matrix chip could be applicable to medical diagnosis of neonatal metabolic disorders. (C) 2017 Elsevier Inc. All rights reserved.
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