期刊
EUROPEAN JOURNAL OF IMMUNOLOGY
卷 45, 期 4, 页码 975-987出版社
WILEY
DOI: 10.1002/eji.201445295
关键词
Bone marrow; CD8 T cell; Gene expression; Interleukin-7; Memory cells
类别
资金
- Deutsche Forschungsgemeinschaft through DFG [1468 IMMUNOBONE]
- European Research Council [268987]
- European Research Council (ERC) [268987] Funding Source: European Research Council (ERC)
- Grants-in-Aid for Scientific Research [25460589, 15H01153] Funding Source: KAKEN
It is believed that memory CD8(+) Tcells are maintained in secondary lymphoid tissues, peripheral tissues, and BM by homeostatic proliferation. Their survival has been shown to be dependent on IL-7, but it is unclear where they acquire it. Here we show that in murine BM, memory CD8(+) Tcells individually colocalize with IL-7(+) reticular stromal cells. The Tcells are resting in terms of global transcription and do not express markers of activation, for example, 4-1BB (CD137), IL-2, or IFN-, despite the expression of CD69 on about 30% of the cells. Ninety-five percent of the memory CD8(+) Tcells in BM are in G(0) phase of cell cycle and do not express Ki-67. Less than 1% is in S/M/G(2) of cell cycle, according to propidium iodide staining. While previous publications have estimated the extent of proliferation of CD8(+) memory Tcells on the basis of BrdU incorporation, we show here that BrdU itself induces proliferation of CD8(+) memory Tcells. Taken together, the present results suggest that CD8(+) memory Tcells are maintained as resting cells in the BM in dedicated niches with their survival conditional on IL-7 receptor signaling.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据