期刊
NATURE STRUCTURAL & MOLECULAR BIOLOGY
卷 24, 期 11, 页码 944-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb.3472
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资金
- EMBO [ALTF 51-2014]
- Swiss National Science Foundation [155515]
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Robertson Foundation
- Alfred P. Sloan Foundation
- Irma T. Hirschl Trust
- Alexandrine and Alexander L. Sinsheimer Fund
- Human Frontier Science Program Development Award
- NIH [1DP2GM123459]
- National Institute of Health [P41GM103314, P41GM109824]
The small-subunit processome represents the earliest stable precursor of the eukaryotic small ribosomal subunit. Here we present the cryo-EM structure of the Saccharomyces cerevisiae small-subunit processome at an overall resolution of 3.8 angstrom, which provides an essentially complete near-atomic model of this assembly. In this nucleolar superstructure, 51 ribosome-assembly factors and two RNAs encapsulate the 18S rRNA precursor and 15 ribosomal proteins in a state that precedes pre-rRNA cleavage at site A1. Extended flexible proteins are employed to connect distant sites in this particle. Molecular mimicry and steric hindrance, as well as protein-and RNA-mediated RNA remodeling, are used in a concerted fashion to prevent the premature formation of the central pseudoknot and its surrounding elements within the small ribosomal subunit.
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