期刊
EUROPEAN JOURNAL OF IMMUNOLOGY
卷 45, 期 9, 页码 2484-2493出版社
WILEY
DOI: 10.1002/eji.201445314
关键词
CLEC-2; Inflammation; Leucocytes; Mouse; Tamoxifen
类别
资金
- Arthritis Research UK [19791]
- British Heart Foundation [CH/03/003, PG/11/119, RG/13/18/30563]
- Medical Research Council
- British Heart Foundation [RG/13/18/30563, PG/11/119/29299] Funding Source: researchfish
- Versus Arthritis [19791] Funding Source: researchfish
Expression of mouse C-type lectin-like receptor 2 (CLEC-2) has been reported on circulating CD11b(high) Gr-1(high) myeloid cells and dendritic cells (DCs) under basal conditions, as well as on a variety of leucocyte subsets following inflammatory stimuli or in vitro cell culture. However, previous studies assessing CLEC-2 expression failed to use CLEC-2-deficient mice as negative controls and instead relied heavily on single antibody clones. Here, we generated CLEC-2-deficient adult mice using two independent approaches and employed two anti-mouse CLEC-2 antibody clones to investigate surface expression on hematopoietic cells from peripheral blood and secondary lymphoid organs. We rule out constitutive CLEC-2 expression on resting DCs and show that CLEC-2 is upregulated in response to LPS-induced systemic inflammation in a small subset of activated DCs isolated from the mesenteric lymph nodes but not the spleen. Moreover, we demonstrate for the first time that peripheral blood B lymphocytes present exogenously derived CLEC-2 and suggest that both circulating B lymphocytes and CD11b(high) Gr-1(high) myeloid cells lose CLEC-2 following entry into secondary lymphoid organs. These results have significant implications for our understanding of CLEC-2 physiological functions
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